|
KMID : 0357920040380030157
|
|
Korean Journal of Pathology
2004 Volume.38 No. 3 p.157 ~ p.164
|
|
|
Expression of Tumor Necrosis Factor-α, Interleukin-1β and Inducible Nitric Oxide Synthase after Stereotaxic Injection of Lipopolysaccharide in Rat Hippocampus
|
|
Oh Hoon-Kyu
Park Ji-Young
Kang Ku-Seong
Kim Jung-Wan
Sohn Yoon-Kyung
Kwak Eun-Kyoung
Kim Ji-Yeon
|
|
|
Abstract
|
|
|
Background:inducible nitric oxide synthase (iNOS) might be detectable in several pathologic conditions, and it is thought to play an important role in their pathophysiology. Tumor necrosis factor (TNF)-α and interleukin (IL)-1β are believed to be essential factors of iNOS induction of the brain.
mothods: After intrahippocampal stereotaxic injection of lipopoly-saccharide (LPS), the rat brains were removed at 6, 12 and 24 h. The rat brain tissues were examined to clarify the expression patterns of TNF-α, IL-1β and iNOS.
Results: The inflammatory cells which were stained with anti-TNF-α antibody, appeared in 6 h and increased for 24 h after LPS injection. The iNOS positive cells appeared after 12 h of LPS injection. A semiquantitative analysis of reverse transcription-polymerase chain reaction (RT-PCR) revealed that the TNF-α and IL-1β mRNA arose at 1 h, peaked at 6 h and then declined until 48 h after LPS injection. The iNOS mRNA arose after 6 h, peaked at 12 h, and declined until 48 h after LPS injection.
Conclusions: We conclude that the induction of inflammatory events by intrahippocampal injection of LPS activates TNF-α and IL-1β secretion, and this is followed by an induction of iNOS expression. TNF-α and IL-1β seem to be related with iNOS expression in brain inflammation.
|
|
|
KEYWORD
|
|
|
Inducible Nitric Oxide Synthase, Tumor Necrosis Factor, Interleukins, Lipopoly, saccharides, Hippocampus
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|